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1. Introduction 2. Overview of Antibiotic Resistance 2.1 Historical background of antibiotic resistance 2.2 Common bacterial pathogens 2.3 Global impact of resistance 3. Metalloenzymes: Definition and Functions 3.1 Classification of metalloenzymes 3.2 Biological roles of metalloenzymes 3.3 Structural characteristics 4. Mechanisms of Antibiotic Resistance 4.1 Genetic mutations and transmission 4.2 Efflux pumps and permeability barriers 4.3 Enzymatic degradation of antibiotics 5. Role of Metalloenzymes in Resistance 5.1 Metallo-beta-lactamases 5.2 Metalloenzymes in aminoglycoside modification 5.3 Metal ion cofactors and enzyme activation 6. Metalloenzyme Inhibition Strategies 6.1 Development of inhibitors 6.2 Combination therapies 6.3 Resistance to metalloenzyme inhibitors 7. Experimental Approaches and Methodologies 7.1 Assays for enzyme activity 7.2 Structural analysis techniques 7.3 Genetic and biochemical studies 8. Future Directions and Implications 8.1 Potential of novel therapies 8.2 Policy and regulatory considerations 8.3 Strategies for overcoming resistance
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